

They filed an opposing trademark for "DOTA" on behalf of DotA-Allstars, LLC, then a subsidiary of their employer, Riot Games, on August 9, 2010. Steve Feak and Steve Mescon expressed their concern that Valve did not have the right to trademark the Dota name, which they believed was a community asset. At Gamescom 2011, Newell said that the trademark was a necessary measure for developing a sequel with the already identifiable brand name.

Shortly after a questions and answers session by IceFrog about the new game, Valve filed a trademark claim. Producer Erik Johnson explained that this was for the reason that the term "Dota" refers to a concept, rather than an acronym. With Valve's acquisition of the franchise, the company adopted the term "Dota", which derives from the original mod's acronym for "Defense of the Ancients". Located in a tarn on the Dire side of the east part of the river is a neutral creep "boss" called "Roshan", who typically requires multiple team members to kill and drops the powerful item "Aegis of Immortality", and significant amounts of gold and experience.

Featured across the map are hostile characters referred to as "neutrals", which are not aligned to either faction and are primarily located in the forests (primarily referred to as the "jungle" in the game, hence the term "jungler" for one who attacks neutral creeps in the jungle). The lanes are guarded by defensive towers, as well as autonomous characters called "creeps", which periodically spawn in groups and travel along the lanes, attacking enemies they encounter. These bases are connected by three paths, referred to as "lanes"-two run alongside the edges of the map, while one runs directly between the two bases. In each stronghold is a critical structure called the "Ancient", a fountain that provide health and lesser structures. These factions are defended by up to five players.

The Radiant is based at the southwest corner of the map, while the Dire is based at the northeast corner the two sides are divided by a river that runs perpendicular to the central lane. Each match of Dota 2 takes place on a functionally symmetrical map that features the strongholds of two warring factions, the Radiant and the Dire.
